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Mouse IGFBP6(Insulin Like Growth Factor Binding Protein 6) Microsample ELISA Kit

Mouse IGFBP6(Insulin Like Growth Factor Binding Protein 6) Microsample ELISA Kit

 
Catalog #
EKEL20149MU
Species
Mouse
Sensitivity
40 pg/mL
Assay Range
78.13-5000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 
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EKEL20149MU-96 2 weeks 96T $575.00
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EKEL20149MU-48 2 weeks 48T $400.00
EKEL20149MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse IGFBP6(Insulin Like Growth Factor Binding Protein 6) Microsample ELISA Kit
Accession (Primary)P47880
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity40 pg/mL
Assay Range78.13-5000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Other Names
IBP6; IGF-binding protein 6
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IGFBP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IGFBP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IGFBP6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
5000.002.0831.987
2500.001.5971.501
1250.001.0750.979
625.000.7850.689
312.500.5650.469
156.250.3170.221
78.130.1900.094
0.000.0960.000
Standard curve: The concentration of IGFBP6 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant IGFBP6 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP6 in samples.

MatrixRecovery rangeAverage
Serum (n=5)85-119%102%
EDTA plasma (n=5)83-116%100%
Heparin plasma (n=5)85-107%96%

Target Information: ( P47880 )

NameIGFBP6
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