Hello, Sign in
My Account
My Account
New customer? Start here
| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL20162HU-96 | 2 weeks | 96T | $575.00 |
Select product before adding to cart
|
||
| EKEL20162HU-48 | 2 weeks | 48T | $400.00 | |||
| EKEL20162HU-96x5 | 2 weeks | 96T*5 | $2,435.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Human CYP21B(Cytochrome P450 21B) ELISA Kit | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accession (Primary) | P08686 | |||||||||||||||||||||||||||
| Reaction Duration | 3.5h | |||||||||||||||||||||||||||
| Target Species | Human | |||||||||||||||||||||||||||
| Reactivity | Human | |||||||||||||||||||||||||||
| Sensitivity | 0.054 ng/mL | |||||||||||||||||||||||||||
| Assay Range | 0.16-10 ng/mL | |||||||||||||||||||||||||||
| Assay type | Sandwich | |||||||||||||||||||||||||||
| Sample type(s) | Tissue homogenates, cell lysates and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| CYP21A2; CPS1; CA21H; CAH1; CYP21; CYP21-B; 21-OHase; Steroid 21-hydroxylase; Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP21B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP21B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP21B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP21B in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
 | ||||||||||||||||||||||||||||
| Precision | ||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||
| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant CYP21B and the recovery rates were calculated by comparing the measured value to the expected amount of CYP21B in samples.
| ||||||||||||||||||||||||||||
Target Information: ( P08686 ) | ||||||||||||||||||||||||||||
| Name | CYP21B | |||||||||||||||||||||||||||
Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
Thank you,
ADMEbio Team
ADMEbio welcomes feedback from our customers.
Thank you,
ADMEbio Team
