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Pig D2D(D-Dimer) ELISA Kit

Pig D2D(D-Dimer) ELISA Kit

 
Catalog #
EKEL20209PC
Species
Pig
Sensitivity
25.4 ng/mL
Assay Range
78.13-5000 ng/mL
Sample type(s)
Plasma
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20209PC-96 2 weeks 96T $735.00
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EKEL20209PC-48 2 weeks 48T $515.00
EKEL20209PC-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NamePig D2D(D-Dimer) ELISA Kit
Reaction Duration3.5h
Target SpeciesPig
ReactivityPig
Sensitivity25.4 ng/mL
Assay Range78.13-5000 ng/mL
Assay typeSandwich
Sample type(s)Plasma
Other Names
D 2 Dimer
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig D2D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig D2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig D2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig D2D in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
5000.002.0751.990
2500.001.6251.540
1250.001.1831.098
625.000.8390.754
312.500.4570.372
156.250.2930.208
78.130.2510.166
0.000.0850.000
Standard curve: The concentration of D2D (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant D2D and the recovery rates were calculated by comparing the measured value to the expected amount of D2D in samples.

MatrixRecovery rangeAverage
Serum (n=5)78-92%85%
EDTA plasma (n=5)87-99%93%
Heparin plasma (n=5)92-107%101%
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