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Rat LOX1(Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit

Rat LOX1(Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit

 
Catalog #
EKEL20211RAT
Species
Rat
Sensitivity
23.5 pg/mL
Assay Range
62.5-4000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20211RAT-96 2 weeks 96T $575.00
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EKEL20211RAT-48 2 weeks 48T $400.00
EKEL20211RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat LOX1(Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
Accession (Primary)O70156
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity23.5 pg/mL
Assay Range62.5-4000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
OLR1; CLEC8A; SCARE1; Oxidized low-density lipoprotein receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; Lectin-type oxidized LDL receptor 1
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LOX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LOX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LOX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LOX1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
4000.002.1192.024
2000.001.5591.464
1000.001.0370.942
500.000.8190.724
250.000.5280.433
125.000.2620.167
62.500.2340.139
0.000.0950.000
Standard curve: The concentration of LOX1 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant LOX1 and the recovery rates were calculated by comparing the measured value to the expected amount of LOX1 in samples.

MatrixRecovery rangeAverage
Serum (n=5)81-95%88%
EDTA plasma (n=5)85-97%91%
Heparin plasma (n=5)96-109%102%

Target Information: ( O70156 )

NameLOX1
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