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Rat TARC(Thymus Activation Regulated Chemokine) Microsample ELISA Kit

Rat TARC(Thymus Activation Regulated Chemokine) Microsample ELISA Kit

 
Catalog #
EKEL20251RAT
Species
Rat
Sensitivity
6.1 pg/mL
Assay Range
15.63-1000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20251RAT-96 2 weeks 96T $575.00
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EKEL20251RAT-48 2 weeks 48T $400.00
EKEL20251RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat TARC(Thymus Activation Regulated Chemokine) Microsample ELISA Kit
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity6.1 pg/mL
Assay Range15.63-1000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Other Names
CCL17; ABCD-2; SCYA17; TARC; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 17; Small-inducible cytokine A17
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TARC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TARC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TARC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TARC in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
1000.002.6012.522
500.001.7811.702
250.001.1351.056
125.000.8730.794
62.500.5090.430
31.250.3860.307
15.630.2350.156
0.000.0790.000
Standard curve: The concentration of TARC (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant TARC and the recovery rates were calculated by comparing the measured value to the expected amount of TARC in samples.

MatrixRecovery rangeAverage
Serum (n=5)93-109%101%
EDTA plasma (n=5)92-113%103%
Heparin plasma (n=5)84-92%88%
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