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Rat Plg(Plasminogen) ELISA Kit

Rat Plg(Plasminogen) ELISA Kit

 
Catalog #
EKEL20260RAT
Species
Rat
Sensitivity
6.8 ng/mL
Assay Range
15.63-1000 ng/mL
Sample type(s)
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20260RAT-96 2 weeks 96T $575.00
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EKEL20260RAT-48 2 weeks 48T $400.00
EKEL20260RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat Plg(Plasminogen) ELISA Kit
Accession (Primary)Q01177
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity6.8 ng/mL
Assay Range15.63-1000 ng/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Other Names
PL; Plasmin; Activation peptide; Angiostatin
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Plg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Plg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Plg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Plg in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
1000.002.2742.186
500.001.6961.608
250.001.1191.031
125.000.9790.891
62.500.5350.447
31.250.3850.297
15.630.2280.140
0.000.0880.000
Standard curve: The concentration of Plg (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant Plg and the recovery rates were calculated by comparing the measured value to the expected amount of Plg in samples.

MatrixRecovery rangeAverage
Serum (n=5)80-97%88%
EDTA plasma (n=5)80-95%87%
Heparin plasma (n=5)83-95%89%

Target Information: ( Q01177 )

NamePlg
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