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Pig APOA2(Apolipoprotein A2) ELISA Kit

Pig APOA2(Apolipoprotein A2) ELISA Kit

 
Catalog #
EKEL20302PC
Species
Pig
Sensitivity
1.9 µg/mL
Assay Range
4.69-300 µg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20302PC-96 2 weeks 96T $735.00
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EKEL20302PC-48 2 weeks 48T $515.00
EKEL20302PC-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NamePig APOA2(Apolipoprotein A2) ELISA Kit
Reaction Duration3.5h
Target SpeciesPig
ReactivityPig
Sensitivity1.9 µg/mL
Assay Range4.69-300 µg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
Apo-A2; Apolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-II
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig APOA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig APOA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig APOA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig APOA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (µg/mL)ODCorrected OD
300.002.4012.317
150.001.6131.529
75.001.1961.112
37.500.8660.782
18.750.5090.425
9.380.3240.240
4.690.1860.102
0.000.0840.000
Standard curve: The concentration of APOA2 (µg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant APOA2 and the recovery rates were calculated by comparing the measured value to the expected amount of APOA2 in samples.

MatrixRecovery rangeAverage
Serum (n=5)88-106%97%
EDTA plasma (n=5)87-99%93%
Heparin plasma (n=5)82-97%90%
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