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Pig IFNa(Interferon Alpha) ELISA Kit

Pig IFNa(Interferon Alpha) ELISA Kit

 
Catalog #
EKEL20310PC
Species
Pig
Sensitivity
6.5 pg/mL
Assay Range
15.63-1000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20310PC-96 2 weeks 96T $735.00
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EKEL20310PC-48 2 weeks 48T $515.00
EKEL20310PC-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NamePig IFNa(Interferon Alpha) ELISA Kit
Accession (Primary)P49879
Reaction Duration3.5h
Target SpeciesPig
ReactivityPig
Sensitivity6.5 pg/mL
Assay Range15.63-1000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Other Names
IFNα1; IFL; LeIF D; IFN; IFN-Alpha; IFNα13; IFN-A; IFNαP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IFNa in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
1000.002.0431.965
500.001.3791.301
250.001.1161.038
125.000.8280.750
62.500.5760.498
31.250.3320.254
15.630.1420.064
0.000.0780.000
Standard curve: The concentration of IFNa (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant IFNa and the recovery rates were calculated by comparing the measured value to the expected amount of IFNa in samples.

MatrixRecovery rangeAverage
Serum (n=5)80-92%86%
EDTA plasma (n=5)85-99%92%
Heparin plasma (n=5)83-97%90%

Target Information: ( P49879 )

NameIFNa
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