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Cattle IL15(Interleukin 15) ELISA Kit

Cattle IL15(Interleukin 15) ELISA Kit

 
Catalog #
EKEL20443BV
Species
Cattle
Sensitivity
6 pg/mL
Assay Range
15.63-1000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20443BV-96 2 weeks 96T $735.00
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EKEL20443BV-48 2 weeks 48T $515.00
EKEL20443BV-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NameCattle IL15(Interleukin 15) ELISA Kit
Accession (Primary)Q28028
Reaction Duration3.5h
Target SpeciesCattle
ReactivityCattle
Sensitivity6 pg/mL
Assay Range15.63-1000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
Il15; Interleukin-15; IL-15
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL15 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
1000.002.1682.081
500.001.5621.475
250.001.1441.057
125.000.8280.741
62.500.5930.506
31.250.3260.239
15.630.1890.102
0.000.0870.000
Standard curve: The concentration of IL15 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant IL15 and the recovery rates were calculated by comparing the measured value to the expected amount of IL15 in samples.

MatrixRecovery rangeAverage
Serum (n=5)92-105%98%
EDTA plasma (n=5)82-95%88%
Heparin plasma (n=5)85-97%91%

Target Information: ( Q28028 )

NameIL15
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