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Pig AP(Aprotinin) ELISA Kit

Pig AP(Aprotinin) ELISA Kit

 
Catalog #
EKEL20472PC
Species
Pig
Sensitivity
0.46 ng/mL
Assay Range
1.57-100 ng/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20472PC-96 2 weeks 96T $735.00
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EKEL20472PC-48 2 weeks 48T $515.00
EKEL20472PC-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NamePig AP(Aprotinin) ELISA Kit
Reaction Duration2h
Target SpeciesPig
ReactivityPig
Sensitivity0.46 ng/mL
Assay Range1.57-100 ng/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma and other biological fluids
Other Names
BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig AP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig AP in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
100.000.227
50.000.386
25.000.596
12.500.812
6.251.156
3.131.511
1.571.775
0.002.226
Standard curve: The concentration of AP (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant AP and the recovery rates were calculated by comparing the measured value to the expected amount of AP in samples.

MatrixRecovery rangeAverage
Serum (n=5)87-99%93%
EDTA plasma (n=5)80-93%86%
Heparin plasma (n=5)93-107%89%
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