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Pig MCP2(Monocyte Chemotactic Protein 2) ELISA Kit

Pig MCP2(Monocyte Chemotactic Protein 2) ELISA Kit

 
Catalog #
EKEL20485PC
Species
Pig
Sensitivity
3.2 pg/mL
Assay Range
7.82-500 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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EKEL20485PC-96 2 weeks 96T $735.00
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EKEL20485PC-48 2 weeks 48T $515.00
EKEL20485PC-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NamePig MCP2(Monocyte Chemotactic Protein 2) ELISA Kit
Accession (Primary)P49873
Reaction Duration3.5h
Target SpeciesPig
ReactivityPig
Sensitivity3.2 pg/mL
Assay Range7.82-500 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
CCL8; CCL-8; SCYA8; MCP-2; HC14; Chemokine C-C-Motif Ligand 8; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 8; Monocyte Chemoattractant Protein 2
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MCP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MCP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MCP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MCP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
500.002.1212.039
250.001.7201.638
125.001.2821.200
62.500.8610.779
31.250.5390.457
15.630.3270.245
7.820.1290.047
0.000.0820.000
Standard curve: The concentration of MCP2 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant MCP2 and the recovery rates were calculated by comparing the measured value to the expected amount of MCP2 in samples.

MatrixRecovery rangeAverage
Serum (n=5)85-97%91%
EDTA plasma (n=5)81-95%88%
Heparin plasma (n=5)90-105%97%

Target Information: ( P49873 )

NameMCP2
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