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Cattle GAL1(Galectin 1) ELISA Kit

Cattle GAL1(Galectin 1) ELISA Kit

 
Catalog #
EKEL20499BV
Species
Cattle
Sensitivity
33 pg/mL
Assay Range
78.13-5000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20499BV-96 2 weeks 96T $735.00
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EKEL20499BV-48 2 weeks 48T $515.00
EKEL20499BV-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NameCattle GAL1(Galectin 1) ELISA Kit
Accession (Primary)P11116
Reaction Duration3.5h
Target SpeciesCattle
ReactivityCattle
Sensitivity33 pg/mL
Assay Range78.13-5000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Other Names
LGALS1; GBP; HLBP14; Galaptin; Lectin, Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle GAL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GAL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle GAL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle GAL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
5000.001.9961.907
2500.001.5931.504
1250.001.1961.107
625.000.7520.663
312.500.5940.505
156.250.3220.233
78.130.1960.107
0.000.0890.000
Standard curve: The concentration of GAL1 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant GAL1 and the recovery rates were calculated by comparing the measured value to the expected amount of GAL1 in samples.

MatrixRecovery rangeAverage
Serum (n=5)87-99%93%
EDTA plasma (n=5)82-95%88%
Heparin plasma (n=5)90-105%97%

Target Information: ( P11116 )

NameGAL1
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