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Goat BMP4(Bone Morphogenetic Protein 4) ELISA Kit

Goat BMP4(Bone Morphogenetic Protein 4) ELISA Kit

 
Catalog #
EKEL20512GT
Species
Goat
Sensitivity
11.9 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20512GT-96 2 weeks 96T $735.00
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EKEL20512GT-48 2 weeks 48T $515.00
EKEL20512GT-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NameGoat BMP4(Bone Morphogenetic Protein 4) ELISA Kit
Reaction Duration3.5h
Target SpeciesGoat
ReactivityGoat
Sensitivity11.9 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat BMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat BMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat BMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.002.0411.950
1000.001.5621.471
500.001.1061.015
250.000.9350.844
125.000.5050.414
62.500.3510.260
31.250.1870.096
0.000.0910.000
Standard curve: The concentration of BMP4 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant BMP4 and the recovery rates were calculated by comparing the measured value to the expected amount of BMP4 in samples.

MatrixRecovery rangeAverage
Serum (n=5)83-95%89%
EDTA plasma (n=5)90-105%97%
Heparin plasma (n=5)80-95%87%
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