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Mouse TAF12(TATA Box Binding Protein Associated Factor 12) ELISA Kit

Mouse TAF12(TATA Box Binding Protein Associated Factor 12) ELISA Kit

 
Catalog #
EKEL20596MU
Species
Mouse
Sensitivity
0.057 ng/mL
Assay Range
0.16-10 ng/mL
Sample type(s)
Tissue homogenates and other biological fluids
 
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EKEL20596MU-96 2 weeks 96T $575.00
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EKEL20596MU-48 2 weeks 48T $400.00
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Product Overview

NameMouse TAF12(TATA Box Binding Protein Associated Factor 12) ELISA Kit
Accession (Primary)Q8VE65
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity0.057 ng/mL
Assay Range0.16-10 ng/mL
Assay typeSandwich
Sample type(s)Tissue homogenates and other biological fluids
Other Names
TAF2J; TAFII20; Transcription Initiation Factor TFIID Subunit 12; Transcription initiation factor TFIID 20/15 kDa subunits
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TAF12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TAF12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TAF12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TAF12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
10.002.0541.965
5.001.5931.504
2.501.1751.086
1.250.8260.737
0.630.5270.438
0.320.3660.277
0.160.1750.086
0.000.0890.000
Standard curve: The concentration of TAF12 (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant TAF12 and the recovery rates were calculated by comparing the measured value to the expected amount of TAF12 in samples.

MatrixRecovery rangeAverage
Serum (n=5)85-97%91%
EDTA plasma (n=5)91-99%95%
Heparin plasma (n=5)80-92%86%

Target Information: ( Q8VE65 )

NameTAF12
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