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Mouse LECT1(Leukocyte Cell Derived Chemotaxin 1) Microsample ELISA Kit

Mouse LECT1(Leukocyte Cell Derived Chemotaxin 1) Microsample ELISA Kit

 
Catalog #
EKEL20658MU
Species
Mouse
Sensitivity
11.1 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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EKEL20658MU-96 2 weeks 96T $575.00
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EKEL20658MU-48 2 weeks 48T $400.00
EKEL20658MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse LECT1(Leukocyte Cell Derived Chemotaxin 1) Microsample ELISA Kit
Accession (Primary)Q9Z1F6
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity11.1 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
BRICD3; CHM-I; CHM1; CH-SP; Chondromodulin-1; Chondrosurfactant protein
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LECT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LECT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LECT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LECT1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.001.9961.902
1000.001.5021.408
500.001.1941.100
250.000.7750.681
125.000.5240.430
62.500.3210.227
31.250.1420.048
0.000.0940.000
Standard curve: The concentration of LECT1 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant LECT1 and the recovery rates were calculated by comparing the measured value to the expected amount of LECT1 in samples.

MatrixRecovery rangeAverage
Serum (n=5)93-117%105%
EDTA plasma (n=5)88-121%105%
Heparin plasma (n=5)83-95%89%

Target Information: ( Q9Z1F6 )

NameLECT1
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