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Mouse REG3g(Regenerating Islet Derived Protein 3 Gamma) ELISA Kit

Mouse REG3g(Regenerating Islet Derived Protein 3 Gamma) ELISA Kit

 
Catalog #
EKEL20708MU
Species
Mouse
Sensitivity
11.3 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20708MU-96 2 weeks 96T $575.00
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EKEL20708MU-48 2 weeks 48T $400.00
EKEL20708MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse REG3g(Regenerating Islet Derived Protein 3 Gamma) ELISA Kit
Accession (Primary)O09049
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity11.3 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
REG-III; PAP1B; PAPIB; UNQ429; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse REG3g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse REG3g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse REG3g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse REG3g in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.002.1682.015
1000.001.6291.476
500.001.1160.963
250.000.8050.652
125.000.5530.400
62.500.3320.179
31.250.2270.074
0.000.1530.000
Standard curve: The concentration of REG3g (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant REG3g and the recovery rates were calculated by comparing the measured value to the expected amount of REG3g in samples.

MatrixRecovery rangeAverage
Serum (n=5)78-92%85%
EDTA plasma (n=5)80-95%87%
Heparin plasma (n=5)78-96%87%

Target Information: ( O09049 )

NameREG3g
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