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Human WNT7A(Wingless Type MMTV Integration Site Family, Member 7A) ELISA Kit

Human WNT7A(Wingless Type MMTV Integration Site Family, Member 7A) ELISA Kit

 
Catalog #
EKEL20780HU
Species
Human
Sensitivity
11.2 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Tissue homogenates, cell lysates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20780HU-96 2 weeks 96T $575.00
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EKEL20780HU-48 2 weeks 48T $400.00
EKEL20780HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman WNT7A(Wingless Type MMTV Integration Site Family, Member 7A) ELISA Kit
Accession (Primary)O00755
Reaction Duration3.5h
Target SpeciesHuman
ReactivityHuman
Sensitivity11.2 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeSandwich
Sample type(s)Tissue homogenates, cell lysates and other biological fluids
Other Names
Proto-Oncogene Wnt7a Protein
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WNT7A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WNT7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WNT7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WNT7A in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.002.1542.068
1000.001.6281.542
500.001.1361.050
250.000.9280.842
125.000.4730.387
62.500.3050.219
31.250.3250.239
0.000.0860.000
Standard curve: The concentration of WNT7A (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant WNT7A and the recovery rates were calculated by comparing the measured value to the expected amount of WNT7A in samples.

MatrixRecovery rangeAverage
Serum (n=5)83-98%90%
EDTA plasma (n=5)80-95%87%
Heparin plasma (n=5)86-99%92%

Target Information: ( O00755 )

NameWNT7A
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