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Human TCIRG1(T-Cell, Immune Regulator 1) Microsample ELISA Kit

Human TCIRG1(T-Cell, Immune Regulator 1) Microsample ELISA Kit

 
Catalog #
EKEL20786HU
Species
Human
Sensitivity
6 pg/mL
Assay Range
15.63-1000 pg/mL
Sample type(s)
Serum and other biological fluids
 
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EKEL20786HU-96 2 weeks 96T $575.00
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EKEL20786HU-48 2 weeks 48T $400.00
EKEL20786HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman TCIRG1(T-Cell, Immune Regulator 1) Microsample ELISA Kit
Accession (Primary)Q13488
Reaction Duration3.5h
Target SpeciesHuman
ReactivityHuman
Sensitivity6 pg/mL
Assay Range15.63-1000 pg/mL
Assay typeSandwich
Sample type(s)Serum and other biological fluids
Other Names
Stv1; Vph1; ATP6N1C; ATP6V0A3; Atp6i; OC-116kDa; OC116; OPTB1; TIRC7; a3; V-Type Proton ATPase 116 kDa Subunit A Isoform 3; Osteoclastic proton pump 116 kDa subunit
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TCIRG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TCIRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TCIRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TCIRG1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
1000.002.3182.218
500.001.5871.487
250.001.1551.055
125.000.8830.783
62.500.6650.565
31.250.3040.204
15.630.2400.140
0.000.1000.000
Standard curve: The concentration of TCIRG1 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant TCIRG1 and the recovery rates were calculated by comparing the measured value to the expected amount of TCIRG1 in samples.

MatrixRecovery rangeAverage
Serum (n=5)85-96%91%
EDTA plasma (n=5)88-98%93%
Heparin plasma (n=5)93-99%96%

Target Information: ( Q13488 )

NameTCIRG1
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