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Human FAM3B(Family With Sequence Similarity 3, Member B) ELISA Kit

Human FAM3B(Family With Sequence Similarity 3, Member B) ELISA Kit

 
Catalog #
EKEL20851HU
Species
Human
Sensitivity
0.257 ng/mL
Assay Range
0.63-40 ng/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20851HU-96 2 weeks 96T $575.00
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EKEL20851HU-48 2 weeks 48T $400.00
EKEL20851HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman FAM3B(Family With Sequence Similarity 3, Member B) ELISA Kit
Accession (Primary)P58499
Reaction Duration3.5h
Target SpeciesHuman
ReactivityHuman
Sensitivity0.257 ng/mL
Assay Range0.63-40 ng/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
ORF9; PANDER; PRED44; C21orf11; C21orf76; Cytokine-like protein 2-21; Pancreatic-derived factor
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FAM3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FAM3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FAM3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FAM3B in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
40.001.9521.848
20.001.5871.483
10.001.1831.079
5.000.8520.748
2.500.5270.423
1.250.3290.225
0.630.2160.112
0.000.1040.000
Standard curve: The concentration of FAM3B (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant FAM3B and the recovery rates were calculated by comparing the measured value to the expected amount of FAM3B in samples.

MatrixRecovery rangeAverage
Serum (n=5)82-94%88%
EDTA plasma (n=5)85-97%91%
Heparin plasma (n=5)82-94%88%

Target Information: ( P58499 )

NameFAM3B
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