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Mouse GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit

Mouse GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit

 
Catalog #
EKEL20944MU
Species
Mouse
Sensitivity
30 pg/mL
Assay Range
78.13-5000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL20944MU-96 2 weeks 96T $575.00
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EKEL20944MU-48 2 weeks 48T $400.00
EKEL20944MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit
Accession (Primary)P56213
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity30 pg/mL
Assay Range78.13-5000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
ERV1; ALR; HERV1; HPO; HPO1; HPO2; HSS; FAD-linked sulfhydryl oxidase ALR; Augmenter of liver regeneration
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GFER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GFER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GFER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GFER in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
5000.002.2682.185
2500.001.5561.473
1250.001.1961.113
625.000.8970.814
312.500.5940.511
156.250.3870.304
78.130.1890.106
0.000.0830.000
Standard curve: The concentration of GFER (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant GFER and the recovery rates were calculated by comparing the measured value to the expected amount of GFER in samples.

MatrixRecovery rangeAverage
Serum (n=5)80-95%87%
EDTA plasma (n=5)87-99%93%
Heparin plasma (n=5)87-99%93%

Target Information: ( P56213 )

NameGFER
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