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Rat INSL3(Insulin Like Protein 3) ELISA Kit

Rat INSL3(Insulin Like Protein 3) ELISA Kit

 
Catalog #
EKEL21007RAT
Species
Rat
Sensitivity
62 pg/mL
Assay Range
234.38-15000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21007RAT-96 2 weeks 96T $575.00
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EKEL21007RAT-48 2 weeks 48T $400.00
EKEL21007RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat INSL3(Insulin Like Protein 3) ELISA Kit
Accession (Primary)Q9WUK0
Reaction Duration2h
Target SpeciesRat
ReactivityRat
Sensitivity62 pg/mL
Assay Range234.38-15000 pg/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma and other biological fluids
Other Names
RLNL; RLF; Relaxin-Like Factor, Leydig Cell; Leydig insulin-like peptide
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat INSL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat INSL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat INSL3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
15000.000.185
7500.000.389
3750.000.621
1875.000.833
937.501.197
468.751.522
234.381.755
0.002.325
Standard curve: The concentration of INSL3 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant INSL3 and the recovery rates were calculated by comparing the measured value to the expected amount of INSL3 in samples.

MatrixRecovery rangeAverage
Serum (n=5)88-99%93%
EDTA plasma (n=5)83-95%89%
Heparin plasma (n=5)83-97%86%

Target Information: ( Q9WUK0 )

NameINSL3
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