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Mouse FAPa(Fibroblast Activation Protein Alpha) ELISA Kit

Mouse FAPa(Fibroblast Activation Protein Alpha) ELISA Kit

 
Catalog #
EKEL21092MU
Species
Mouse
Sensitivity
13.1 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21092MU-96 2 weeks 96T $575.00
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EKEL21092MU-48 2 weeks 48T $400.00
EKEL21092MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse FAPa(Fibroblast Activation Protein Alpha) ELISA Kit
Accession (Primary)P97321
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity13.1 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Other Names
APCE; SIMP; DPPIV; Seprase; 170 kDa melanoma membrane-bound gelatinase; Post-proline cleaving enzyme; Serine integral membrane protease; Surface-expressed protease
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FAPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FAPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FAPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FAPa in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.002.1052.020
1000.001.6151.530
500.001.2251.140
250.000.9630.878
125.000.5190.434
62.500.3050.220
31.250.2560.171
0.000.0850.000
Standard curve: The concentration of FAPa (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant FAPa and the recovery rates were calculated by comparing the measured value to the expected amount of FAPa in samples.

MatrixRecovery rangeAverage
Serum (n=5)85-99%92%
EDTA plasma (n=5)83-97%90%
Heparin plasma (n=5)80-92%86%

Target Information: ( P97321 )

NameFAPa
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