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Rat MAP3K6(Mitogen Activated Protein Kinase Kinase Kinase 6) ELISA Kit

Rat MAP3K6(Mitogen Activated Protein Kinase Kinase Kinase 6) ELISA Kit

 
Catalog #
EKEL21112RAT
Species
Rat
Sensitivity
0.112 ng/mL
Assay Range
0.32-20 ng/mL
Sample type(s)
Tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21112RAT-96 2 weeks 96T $575.00
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EKEL21112RAT-48 2 weeks 48T $400.00
EKEL21112RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat MAP3K6(Mitogen Activated Protein Kinase Kinase Kinase 6) ELISA Kit
Accession (Primary)D3ZFL3
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity0.112 ng/mL
Assay Range0.32-20 ng/mL
Assay typeSandwich
Sample type(s)Tissue homogenates and other biological fluids
Other Names
MAPKKK6; ASK2; MEKK6; Apoptosis Signal Regulating Kinase 2
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAP3K6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAP3K6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAP3K6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAP3K6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
20.002.2012.108
10.001.6741.581
5.001.1391.046
2.500.7970.704
1.250.5070.414
0.630.3350.242
0.320.1960.103
0.000.0930.000
Standard curve: The concentration of MAP3K6 (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant MAP3K6 and the recovery rates were calculated by comparing the measured value to the expected amount of MAP3K6 in samples.

MatrixRecovery rangeAverage
Serum (n=5)82-98%90%
EDTA plasma (n=5)84-98%91%
Heparin plasma (n=5)80-96%88%

Target Information: ( D3ZFL3 )

NameMAP3K6
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