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Rat ELA2A(Elastase 2A) ELISA Kit

Rat ELA2A(Elastase 2A) ELISA Kit

 
Catalog #
EKEL21269RAT
Species
Rat
Sensitivity
21.9 pg/mL
Assay Range
62.5-4000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21269RAT-96 2 weeks 96T $575.00
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EKEL21269RAT-48 2 weeks 48T $400.00
EKEL21269RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat ELA2A(Elastase 2A) ELISA Kit
Accession (Primary)P00774
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity21.9 pg/mL
Assay Range62.5-4000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
ELA1; PE-1; CELA2A; Chymotrypsin-Like Elastase Family Member 2A
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ELA2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ELA2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ELA2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ELA2A in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
4000.002.1262.028
2000.001.5931.495
1000.001.1391.041
500.000.8260.728
250.000.5410.443
125.000.3230.225
62.500.2060.108
0.000.0980.000
Standard curve: The concentration of ELA2A (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant ELA2A and the recovery rates were calculated by comparing the measured value to the expected amount of ELA2A in samples.

MatrixRecovery rangeAverage
Serum (n=5)88-101%94%
EDTA plasma (n=5)91-105%98%
Heparin plasma (n=5)85-97%91%

Target Information: ( P00774 )

NameELA2A
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