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Mouse PLUNC(Palate/Lung And Nasal Epithelium Associated Protein) ELISA Kit

Mouse PLUNC(Palate/Lung And Nasal Epithelium Associated Protein) ELISA Kit

 
Catalog #
EKEL21302MU
Species
Mouse
Sensitivity
7 pg/mL
Assay Range
15.63-1000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21302MU-96 2 weeks 96T $575.00
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EKEL21302MU-48 2 weeks 48T $400.00
EKEL21302MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse PLUNC(Palate/Lung And Nasal Epithelium Associated Protein) ELISA Kit
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity7 pg/mL
Assay Range15.63-1000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
BPIFA1; SPURT; NASG; LUNX; SPLUNC1; Secretory Protein In Upper Respiratory Tracts; BPI Fold Containing Family A, Member 1; Nasopharyngeal carcinoma-related
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PLUNC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PLUNC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PLUNC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PLUNC in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
1000.001.9461.838
500.001.6281.520
250.001.1351.027
125.000.9330.825
62.500.5890.481
31.250.3150.207
15.630.2150.107
0.000.1080.000
Standard curve: The concentration of PLUNC (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant PLUNC and the recovery rates were calculated by comparing the measured value to the expected amount of PLUNC in samples.

MatrixRecovery rangeAverage
Serum (n=5)89-107%98%
EDTA plasma (n=5)87-99%93%
Heparin plasma (n=5)78-90%84%
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