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Human RBM20(RNA Binding Motif Protein 20) ELISA Kit

Human RBM20(RNA Binding Motif Protein 20) ELISA Kit

 
Catalog #
EKEL21313HU
Species
Human
Sensitivity
0.113 ng/mL
Assay Range
0.32-20 ng/mL
Sample type(s)
Tissue homogenates, cell lysates and other biological fluids
 
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EKEL21313HU-96 2 weeks 96T $575.00
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EKEL21313HU-48 2 weeks 48T $400.00
EKEL21313HU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameHuman RBM20(RNA Binding Motif Protein 20) ELISA Kit
Accession (Primary)Q5T481
Reaction Duration3.5h
Target SpeciesHuman
ReactivityHuman
Sensitivity0.113 ng/mL
Assay Range0.32-20 ng/mL
Assay typeSandwich
Sample type(s)Tissue homogenates, cell lysates and other biological fluids
Other Names
Rbm20; RBM20
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RBM20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RBM20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RBM20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RBM20 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
20.002.0751.980
10.001.7841.689
5.001.2151.120
2.500.9210.826
1.250.5190.424
0.630.3020.207
0.320.2150.120
0.000.0950.000
Standard curve: The concentration of RBM20 (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant RBM20 and the recovery rates were calculated by comparing the measured value to the expected amount of RBM20 in samples.

MatrixRecovery rangeAverage
Serum (n=5)78-93%85%
EDTA plasma (n=5)87-99%93%
Heparin plasma (n=5)87-95%91%

Target Information: ( Q5T481 )

NameRBM20
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