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Mouse CER(Cerberus) ELISA Kit

Mouse CER(Cerberus) ELISA Kit

 
Catalog #
EKEL21392MU
Species
Mouse
Sensitivity
11.7 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21392MU-96 2 weeks 96T $575.00
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EKEL21392MU-48 2 weeks 48T $400.00
EKEL21392MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse CER(Cerberus) ELISA Kit
Accession (Primary)O55233
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity11.7 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
CER1; DAND4; Cerberus 1; DAN domain family member 4; Cysteine Knot Superfamily, Homolog(Xenopus Laevis); Cerberus-related protein
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CER in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.002.1262.022
1000.001.5021.398
500.001.1981.094
250.000.7520.648
125.000.5470.443
62.500.3350.231
31.250.2160.112
0.000.1040.000
Standard curve: The concentration of CER (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant CER and the recovery rates were calculated by comparing the measured value to the expected amount of CER in samples.

MatrixRecovery rangeAverage
Serum (n=5)82-94%88%
EDTA plasma (n=5)82-98%90%
Heparin plasma (n=5)80-97%88%

Target Information: ( O55233 )

NameCER
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