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Mouse SFRP2(Secreted Frizzled Related Protein 2) ELISA Kit

Mouse SFRP2(Secreted Frizzled Related Protein 2) ELISA Kit

 
Catalog #
EKEL21473MU
Species
Mouse
Sensitivity
44 pg/mL
Assay Range
125-8000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21473MU-96 2 weeks 96T $575.00
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EKEL21473MU-48 2 weeks 48T $400.00
EKEL21473MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse SFRP2(Secreted Frizzled Related Protein 2) ELISA Kit
Accession (Primary)P97299
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity44 pg/mL
Assay Range125-8000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
FRP-2; SARP1; SDF-5; Secreted apoptosis-related protein 1
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SFRP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SFRP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SFRP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SFRP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
8000.002.1192.038
4000.001.6381.557
2000.001.1281.047
1000.000.7350.654
500.000.5930.512
250.000.3150.234
125.000.2880.207
0.000.0810.000
Standard curve: The concentration of SFRP2 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant SFRP2 and the recovery rates were calculated by comparing the measured value to the expected amount of SFRP2 in samples.

MatrixRecovery rangeAverage
Serum (n=5)87-95%91%
EDTA plasma (n=5)78-92%85%
Heparin plasma (n=5)92-106%99%

Target Information: ( P97299 )

NameSFRP2
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