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Dog CCK(Cholecystokinin) ELISA Kit

Dog CCK(Cholecystokinin) ELISA Kit

 
Catalog #
EKEL21542CN
Species
Dog
Sensitivity
4.73 pg/mL
Assay Range
15.63-1000 pg/mL
Sample type(s)
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21542CN-96 2 weeks 96T $735.00
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EKEL21542CN-48 2 weeks 48T $515.00
EKEL21542CN-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NameDog CCK(Cholecystokinin) ELISA Kit
Accession (Primary)Q9TS44
Reaction Duration2h
Target SpeciesDog
ReactivityDog
Sensitivity4.73 pg/mL
Assay Range15.63-1000 pg/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Other Names
CCK-PZ
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog CCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CCK in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
1000.000.197
500.000.413
250.000.722
125.000.993
62.501.236
31.251.589
15.631.887
0.002.057
Standard curve: The concentration of CCK (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant CCK and the recovery rates were calculated by comparing the measured value to the expected amount of CCK in samples.

MatrixRecovery rangeAverage
Serum (n=5)79-93%86%
EDTA plasma (n=5)95-107%101%
Heparin plasma (n=5)85-95%88%

Target Information: ( Q9TS44 )

NameCCK
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