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Rat FGFR1(Fibroblast Growth Factor Receptor 1) ELISA Kit

Rat FGFR1(Fibroblast Growth Factor Receptor 1) ELISA Kit

 
Catalog #
EKEL21544RAT
Species
Rat
Sensitivity
0.121 ng/mL
Assay Range
0.32-20 ng/mL
Sample type(s)
Tissue homogenates, cell lysates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21544RAT-96 2 weeks 96T $575.00
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EKEL21544RAT-48 2 weeks 48T $400.00
EKEL21544RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat FGFR1(Fibroblast Growth Factor Receptor 1) ELISA Kit
Accession (Primary)Q04589
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity0.121 ng/mL
Assay Range0.32-20 ng/mL
Assay typeSandwich
Sample type(s)Tissue homogenates, cell lysates and other biological fluids
Other Names
CD331; FLT2, KAL2; CEK; FLG; BFGFR, N-SAM, Basic Fibroblast Growth Factor Receptor 1; Fms-Related Tyrosine Kinase-2; Pfeiffer Syndrome
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGFR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGFR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGFR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGFR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
20.001.9501.851
10.001.3871.288
5.001.1351.036
2.500.9330.834
1.250.5170.418
0.630.3870.288
0.320.2350.136
0.000.0990.000
Standard curve: The concentration of FGFR1 (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant FGFR1 and the recovery rates were calculated by comparing the measured value to the expected amount of FGFR1 in samples.

MatrixRecovery rangeAverage
Serum (n=5)82-98%90%
EDTA plasma (n=5)80-97%88%
Heparin plasma (n=5)82-94%88%

Target Information: ( Q04589 )

NameFGFR1
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