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Rat NPR1(Natriuretic Peptide Receptor 1) ELISA Kit

Rat NPR1(Natriuretic Peptide Receptor 1) ELISA Kit

 
Catalog #
EKEL21556RAT
Species
Rat
Sensitivity
0.105 ng/mL
Assay Range
0.32-20 ng/mL
Sample type(s)
Tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21556RAT-96 2 weeks 96T $575.00
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EKEL21556RAT-48 2 weeks 48T $400.00
EKEL21556RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat NPR1(Natriuretic Peptide Receptor 1) ELISA Kit
Accession (Primary)P18910
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity0.105 ng/mL
Assay Range0.32-20 ng/mL
Assay typeSandwich
Sample type(s)Tissue homogenates and other biological fluids
Other Names
ANPRA; ANPa; GUC2A; GUCY2A; NPR-A; Guanylate Cyclase A; Atrionatriuretic Peptide Receptor A; Atrial natriuretic peptide receptor type A
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NPR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NPR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
20.002.1542.073
10.001.5931.512
5.001.2231.142
2.500.7550.674
1.250.5080.427
0.630.3190.238
0.320.2880.207
0.000.0810.000
Standard curve: The concentration of NPR1 (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant NPR1 and the recovery rates were calculated by comparing the measured value to the expected amount of NPR1 in samples.

MatrixRecovery rangeAverage
Serum (n=5)82-95%88%
EDTA plasma (n=5)94-107%100%
Heparin plasma (n=5)87-95%91%

Target Information: ( P18910 )

NameNPR1
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