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Mouse PRDX6(Peroxiredoxin 6) ELISA Kit

Mouse PRDX6(Peroxiredoxin 6) ELISA Kit

 
Catalog #
EKEL21583MU
Species
Mouse
Sensitivity
30 pg/mL
Assay Range
78.13-5000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21583MU-96 2 weeks 96T $575.00
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EKEL21583MU-48 2 weeks 48T $400.00
EKEL21583MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse PRDX6(Peroxiredoxin 6) ELISA Kit
Accession (Primary)O08709
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity30 pg/mL
Assay Range78.13-5000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
1-Cys; AOP2; NSGPx; PRX; aiPLA2; p29; Acidic calcium-independent phospholipase A2; Antioxidant protein 2; Non-selenium glutathione peroxidase; Red blood cells page spot 12
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRDX6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRDX6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRDX6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRDX6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
5000.001.9521.849
2500.001.5271.424
1250.001.1771.074
625.000.9330.830
312.500.5320.429
156.250.3050.202
78.130.2570.154
0.000.1030.000
Standard curve: The concentration of PRDX6 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant PRDX6 and the recovery rates were calculated by comparing the measured value to the expected amount of PRDX6 in samples.

MatrixRecovery rangeAverage
Serum (n=5)85-97%91%
EDTA plasma (n=5)83-99%95%
Heparin plasma (n=5)85-97%91%

Target Information: ( O08709 )

NamePRDX6
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