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Rat GHRL(Ghrelin) ELISA Kit

Rat GHRL(Ghrelin) ELISA Kit

 
Catalog #
EKEL21629RAT
Species
Rat
Sensitivity
9.85 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21629RAT-96 2 weeks 96T $575.00
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EKEL21629RAT-48 2 weeks 48T $400.00
EKEL21629RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat GHRL(Ghrelin) ELISA Kit
Accession (Primary)Q9QYH7
Reaction Duration2h
Target SpeciesRat
ReactivityRat
Sensitivity9.85 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma and other biological fluids
Other Names
MTLRP; Preproghrelin; Growth Hormone Secretagogue Receptor Ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.000.215
1000.000.336
500.000.451
250.000.899
125.001.139
62.501.537
31.251.733
0.002.028
Standard curve: The concentration of GHRL (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant GHRL and the recovery rates were calculated by comparing the measured value to the expected amount of GHRL in samples.

MatrixRecovery rangeAverage
Serum (n=5)86-98%92%
EDTA plasma (n=5)95-107%101%
Heparin plasma (n=5)83-95%86%

Target Information: ( Q9QYH7 )

NameGHRL
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