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Mouse PLA2G2A(Phospholipase A2, Group IIA) ELISA Kit

Mouse PLA2G2A(Phospholipase A2, Group IIA) ELISA Kit

 
Catalog #
EKEL21672MU
Species
Mouse
Sensitivity
6.4 pg/mL
Assay Range
15.63-1000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21672MU-96 2 weeks 96T $575.00
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EKEL21672MU-48 2 weeks 48T $400.00
EKEL21672MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse PLA2G2A(Phospholipase A2, Group IIA) ELISA Kit
Accession (Primary)P31482
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity6.4 pg/mL
Assay Range15.63-1000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
PLA2; MOM1; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2(platelets, synovial fluid); Non-pancreatic secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PLA2G2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PLA2G2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PLA2G2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PLA2G2A in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
1000.002.1152.027
500.001.5021.414
250.001.1741.086
125.000.7260.638
62.500.5870.499
31.250.2390.151
15.630.1240.036
0.000.0880.000
Standard curve: The concentration of PLA2G2A (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant PLA2G2A and the recovery rates were calculated by comparing the measured value to the expected amount of PLA2G2A in samples.

MatrixRecovery rangeAverage
Serum (n=5)88-102%95%
EDTA plasma (n=5)83-96%89%
Heparin plasma (n=5)92-107%99%

Target Information: ( P31482 )

NamePLA2G2A
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