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Rat TFF1(Trefoil Factor 1) ELISA Kit

Rat TFF1(Trefoil Factor 1) ELISA Kit

 
Catalog #
EKEL21758RAT
Species
Rat
Sensitivity
27 pg/mL
Assay Range
78.13-5000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL21758RAT-96 2 weeks 96T $575.00
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EKEL21758RAT-48 2 weeks 48T $400.00
EKEL21758RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat TFF1(Trefoil Factor 1) ELISA Kit
Accession (Primary)Q63467
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity27 pg/mL
Assay Range78.13-5000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
BCEI; HP1.A; HPS2; PS2; PNR2; Breast Cancer, Estrogen-Inducible Sequence Expressed In; Breast cancer estrogen-inducible protein; Polypeptide P1.A
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
5000.002.1192.023
2500.001.5271.431
1250.001.2361.140
625.000.8150.719
312.500.5930.497
156.250.3290.233
78.130.1970.101
0.000.0960.000
Standard curve: The concentration of TFF1 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant TFF1 and the recovery rates were calculated by comparing the measured value to the expected amount of TFF1 in samples.

MatrixRecovery rangeAverage
Serum (n=5)86-99%92%
EDTA plasma (n=5)80-95%87%
Heparin plasma (n=5)90-105%97%

Target Information: ( Q63467 )

NameTFF1
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