| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL21792RAT-96 | 2 weeks | 96T | $575.00 |
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| EKEL21792RAT-48 | 2 weeks | 48T | $400.00 | |||
| EKEL21792RAT-96x5 | 2 weeks | 96T*5 | $2,435.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Rat PCSK9(Proprotein Convertase Subtilisin/Kexin Type 9) Microsample ELISA Kit | |||||||||||||||||||||||||||
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| Accession (Primary) | P59996 | |||||||||||||||||||||||||||
| Reaction Duration | 3.5h | |||||||||||||||||||||||||||
| Target Species | Rat | |||||||||||||||||||||||||||
| Reactivity | Rat | |||||||||||||||||||||||||||
| Sensitivity | 1.17 ng/mL | |||||||||||||||||||||||||||
| Assay Range | 3.13-200 ng/mL | |||||||||||||||||||||||||||
| Assay type | Sandwich | |||||||||||||||||||||||||||
| Sample type(s) | Serum, plasma, tissue homogenates and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| FH3; HCHOLA3; NARC1; Hypercholesterolemia, Autosomal Dominant 3; Neural apoptosis-regulated convertase 1; Proprotein convertase 9; Subtilisin/kexin-like protease PC9 | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PCSK9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PCSK9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PCSK9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PCSK9 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
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| Precision | ||||||||||||||||||||||||||||
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| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant PCSK9 and the recovery rates were calculated by comparing the measured value to the expected amount of PCSK9 in samples.
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Target Information: ( P59996 ) | ||||||||||||||||||||||||||||
| Name | PCSK9 | |||||||||||||||||||||||||||
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