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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL21853MU-96 | 2 weeks | 96T | $575.00 |
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| EKEL21853MU-48 | 2 weeks | 48T | $400.00 | |||
| EKEL21853MU-96x5 | 2 weeks | 96T*5 | $2,435.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Mouse LIPA(Lipase A, Lysosomal Acid) ELISA Kit | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accession (Primary) | Q9Z0M5 | |||||||||||||||||||||||||||
| Reaction Duration | 3.5h | |||||||||||||||||||||||||||
| Target Species | Mouse | |||||||||||||||||||||||||||
| Reactivity | Mouse | |||||||||||||||||||||||||||
| Sensitivity | 0.143 ng/mL | |||||||||||||||||||||||||||
| Assay Range | 0.32-20 ng/mL | |||||||||||||||||||||||||||
| Assay type | Sandwich | |||||||||||||||||||||||||||
| Sample type(s) | Serum, plasma, tissue homogenates and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| LAL; CESD; Lipase A, Lysosomal Acid, Cholesterol Esterase(Wolman Disease); Acid Cholesteryl Ester Hydrolase; Lysosomal acid lipase/cholesteryl ester hydrolase | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LIPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LIPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LIPA in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
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| Precision | ||||||||||||||||||||||||||||
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| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant LIPA and the recovery rates were calculated by comparing the measured value to the expected amount of LIPA in samples.
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Target Information: ( Q9Z0M5 ) | ||||||||||||||||||||||||||||
| Name | LIPA | |||||||||||||||||||||||||||
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ADMEbio Team
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ADMEbio Team
