0
There are 0 item in your cart
Cart Subtotal $0.00 Checkout
>   Home   >   Products   >   ELISA Kits   >   Rat RLN(Relaxin) ELISA Kit   

Rat RLN(Relaxin) ELISA Kit

Rat RLN(Relaxin) ELISA Kit

 
Catalog #
EKEL22001RAT
Species
Rat
Sensitivity
8.85 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
Be the first to write a review
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL22001RAT-96 2 weeks 96T $575.00
Select product before adding to cart
EKEL22001RAT-48 2 weeks 48T $400.00
EKEL22001RAT-96x5 2 weeks 96T*5 $2,435.00
  • Datasheet
  • Citations
  • Reviews

Product Overview

NameRat RLN(Relaxin) ELISA Kit
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity8.85 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma and other biological fluids
Other Names
RLXH1; Relaxin H1; Prorelaxin H1
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RLN in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.002.1462.074
1000.001.6281.556
500.001.2121.140
250.000.8050.733
125.000.6320.560
62.500.3350.263
31.250.1700.098
0.000.0720.000
Standard curve: The concentration of RLN (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant RLN and the recovery rates were calculated by comparing the measured value to the expected amount of RLN in samples.

MatrixRecovery rangeAverage
Serum (n=5)84-92%87%
EDTA plasma (n=5)95-104%98%
Heparin plasma (n=5)82-90%86%
Citation banner

Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Thank you,
ADMEbio Team

Citation banner
Be the first to review product

ADMEbio welcomes feedback from our customers.

  • Be seen as an expert in the field of the products you are reviewing
  • Showcase your results (you can upload an image) to the antibody community. New product applications are welcome!
  • Share your experience to help other researchers make more informed decisions.
  • Easy process through our 5 star rating system and single login through your account.

Thank you,
ADMEbio Team






Order Information
Call
760-390-3989
Shipping Information
For shipping outside United State, please contact
sales@admebio.com