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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL22019RAT-96 | 2 weeks | 96T | $575.00 |
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| EKEL22019RAT-48 | 2 weeks | 48T | $400.00 | |||
| EKEL22019RAT-96x5 | 2 weeks | 96T*5 | $2,435.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Rat ROS1(C-Ros Oncogene 1, Receptor Tyrosine Kinase) ELISA Kit | |||||||||||||||||||||||||||
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| Accession (Primary) | Q63132 | |||||||||||||||||||||||||||
| Reaction Duration | 3.5h | |||||||||||||||||||||||||||
| Target Species | Rat | |||||||||||||||||||||||||||
| Reactivity | Rat | |||||||||||||||||||||||||||
| Sensitivity | 6.5 pg/mL | |||||||||||||||||||||||||||
| Assay Range | 15.63-1000 pg/mL | |||||||||||||||||||||||||||
| Assay type | Sandwich | |||||||||||||||||||||||||||
| Sample type(s) | Serum, plasma, tissue homogenates, cell lysates and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| MCF3; ROS; c-Ros-1; v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1 | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ROS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ROS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ROS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ROS1 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
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| Precision | ||||||||||||||||||||||||||||
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| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant ROS1 and the recovery rates were calculated by comparing the measured value to the expected amount of ROS1 in samples.
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Target Information: ( Q63132 ) | ||||||||||||||||||||||||||||
| Name | ROS1 | |||||||||||||||||||||||||||
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ADMEbio Team
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ADMEbio Team
