0
There are 0 item in your cart
Cart Subtotal $0.00 Checkout

Rat SLC7A11(Solute Carrier Family 7, Member 11) ELISA Kit

Rat SLC7A11(Solute Carrier Family 7, Member 11) ELISA Kit

 
Catalog #
EKEL22051RAT
Species
Rat
Sensitivity
0.54 ng/mL
Assay Range
1.57-100 ng/mL
Sample type(s)
Tissue homogenates, cell lysates and other biological fluids
 
Be the first to write a review
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL22051RAT-96 2 weeks 96T $575.00
Select product before adding to cart
EKEL22051RAT-48 2 weeks 48T $400.00
EKEL22051RAT-96x5 2 weeks 96T*5 $2,435.00
  • Datasheet
  • Citations
  • Reviews

Product Overview

NameRat SLC7A11(Solute Carrier Family 7, Member 11) ELISA Kit
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity0.54 ng/mL
Assay Range1.57-100 ng/mL
Assay typeSandwich
Sample type(s)Tissue homogenates, cell lysates and other biological fluids
Other Names
CCBR1; xCT; Cystine/Glutamate Transporter, Cationic Amino Acid Transporter, y+ System; Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SLC7A11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SLC7A11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SLC7A11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SLC7A11 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
100.001.9411.874
50.001.5001.433
25.001.1761.109
12.500.7280.661
6.250.4570.390
3.130.3360.269
1.570.2260.159
0.000.0670.000
Standard curve: The concentration of SLC7A11 (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant SLC7A11 and the recovery rates were calculated by comparing the measured value to the expected amount of SLC7A11 in samples.

MatrixRecovery rangeAverage
Serum (n=5)88-102%95%
EDTA plasma (n=5)90-105%97%
Heparin plasma (n=5)80-92%86%
Citation banner

Thousands of laboratories across the world have published research that depended on the performance of antibodies from ADMEbio to advance their research.
Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Thank you,
ADMEbio Team

Citation banner
Be the first to review product

ADMEbio welcomes feedback from our customers.

  • Be seen as an expert in the field of the products you are reviewing
  • Showcase your results (you can upload an image) to the antibody community. New product applications are welcome!
  • Share your experience to help other researchers make more informed decisions.
  • Easy process through our 5 star rating system and single login through your account.

Thank you,
ADMEbio Team






Order Information
Call
760-390-3989
Shipping Information
For shipping outside United State, please contact
sales@admebio.com