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Mouse MYH7(Myosin Heavy Chain 7, Cardiac Muscle, Beta) ELISA Kit

Mouse MYH7(Myosin Heavy Chain 7, Cardiac Muscle, Beta) ELISA Kit

 
Catalog #
EKEL22064MU
Species
Mouse
Sensitivity
12.9 pg/mL
Assay Range
31.25-2000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL22064MU-96 2 weeks 96T $575.00
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EKEL22064MU-48 2 weeks 48T $400.00
EKEL22064MU-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameMouse MYH7(Myosin Heavy Chain 7, Cardiac Muscle, Beta) ELISA Kit
Accession (Primary)Q91Z83
Reaction Duration3.5h
Target SpeciesMouse
ReactivityMouse
Sensitivity12.9 pg/mL
Assay Range31.25-2000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
CMD1S; CMH1; MPD1; MYHCB; Myopathy, Distal 1; Myosin heavy chain slow isoform; Myosin heavy chain, cardiac muscle beta isoform
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MYH7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MYH7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MYH7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MYH7 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
2000.001.9081.826
1000.001.5271.445
500.001.0750.993
250.000.7820.700
125.000.4440.362
62.500.3250.243
31.250.1960.114
0.000.0820.000
Standard curve: The concentration of MYH7 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant MYH7 and the recovery rates were calculated by comparing the measured value to the expected amount of MYH7 in samples.

MatrixRecovery rangeAverage
Serum (n=5)81-93%87%
EDTA plasma (n=5)82-94%88%
Heparin plasma (n=5)85-97%91%

Target Information: ( Q91Z83 )

NameMYH7
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