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Rat TCN2(Transcobalamin II, Macrocytic Anemia) ELISA Kit

Rat TCN2(Transcobalamin II, Macrocytic Anemia) ELISA Kit

 
Catalog #
EKEL22169RAT
Species
Rat
Sensitivity
30 pg/mL
Assay Range
78.13-5000 pg/mL
Sample type(s)
Serum, plasma, tissue homogenates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL22169RAT-96 2 weeks 96T $575.00
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EKEL22169RAT-48 2 weeks 48T $400.00
EKEL22169RAT-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameRat TCN2(Transcobalamin II, Macrocytic Anemia) ELISA Kit
Accession (Primary)Q9R0D6
Reaction Duration3.5h
Target SpeciesRat
ReactivityRat
Sensitivity30 pg/mL
Assay Range78.13-5000 pg/mL
Assay typeSandwich
Sample type(s)Serum, plasma, tissue homogenates and other biological fluids
Other Names
TC2; Transcobalamin II
Principle of the Assay
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TCN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TCN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TCN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TCN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
5000.002.3792.288
2500.001.6361.545
1250.001.2231.132
625.000.8150.724
312.500.6110.520
156.250.3520.261
78.130.2080.117
0.000.0910.000
Standard curve: The concentration of TCN2 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant TCN2 and the recovery rates were calculated by comparing the measured value to the expected amount of TCN2 in samples.

MatrixRecovery rangeAverage
Serum (n=5)90-105%97%
EDTA plasma (n=5)87-99%93%
Heparin plasma (n=5)82-95%88%

Target Information: ( Q9R0D6 )

NameTCN2
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