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Rabbit HB(Hemoglobin) ELISA Kit

Rabbit HB(Hemoglobin) ELISA Kit

 
Catalog #
EKEL22474RB
Species
Rabbit
Sensitivity
0.55 µg/mL
Assay Range
1.57-100 µg/mL
Sample type(s)
Serum, plasma, erythrocyte lysates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL22474RB-96 2 weeks 96T $735.00
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EKEL22474RB-48 2 weeks 48T $515.00
EKEL22474RB-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NameRabbit HB(Hemoglobin) ELISA Kit
Reaction Duration2h
Target SpeciesRabbit
ReactivityRabbit
Sensitivity0.55 µg/mL
Assay Range1.57-100 µg/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma, erythrocyte lysates and other biological fluids
Other Names
Hgb; Haemoglobin; Heterotetramer(αβ)2
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit HB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit HB in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (µg/mL)ODCorrected OD
100.000.212
50.000.386
25.000.575
12.500.823
6.251.112
3.131.537
1.571.796
0.002.112
Standard curve: The concentration of HB (µg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant HB and the recovery rates were calculated by comparing the measured value to the expected amount of HB in samples.

MatrixRecovery rangeAverage
Serum (n=5)80-93%86%
EDTA plasma (n=5)93-107%100%
Heparin plasma (n=5)87-99%85%
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