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Chicken IL17(Interleukin 17) ELISA Kit

Chicken IL17(Interleukin 17) ELISA Kit

 
Catalog #
EKEL22475CK
Species
Chicken
Sensitivity
5.2 pg/mL
Assay Range
15.63-1000 pg/mL
Sample type(s)
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL22475CK-96 2 weeks 96T $735.00
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EKEL22475CK-48 2 weeks 48T $515.00
EKEL22475CK-96x5 2 weeks 96T*5 $3,125.00
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Product Overview

NameChicken IL17(Interleukin 17) ELISA Kit
Reaction Duration2h
Target SpeciesChicken
ReactivityChicken
Sensitivity5.2 pg/mL
Assay Range15.63-1000 pg/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Other Names
IL-17; IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken IL17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IL17 in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
1000.000.227
500.000.397
250.000.596
125.000.812
62.501.157
31.251.592
15.631.697
0.002.213
Standard curve: The concentration of IL17 (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant IL17 and the recovery rates were calculated by comparing the measured value to the expected amount of IL17 in samples.

MatrixRecovery rangeAverage
Serum (n=5)83-97%90%
EDTA plasma (n=5)78-92%85%
Heparin plasma (n=5)80-95%93%
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