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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0067-5 | 7 days | 5 µg | $120.00 |
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| GRF0067-20 | 7 days | 20 µg | $290.00 | |||
| GRF0067-1 | 7 days | 1 mg | $4,320.00 |
Product Overview | |
| Name | FGF 2 Human, Thermostable |
|---|---|
| Description | |
| Fibroblast Growth Factor-basic Human Recombinant, Thermostable | |
| Accession (Primary) | P09038 |
| Synonyms | |
| HBGF-2, Prostatropin, FGF-2, FGB-b. | |
| Introduction | |
| FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. The binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| FGF-b was lyophilized from 5mM Na 2 PO 4 , pH7.5 and 50mM NaCl. | |
| Stability | |
| Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-basic should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 95.0% as determined by SDS-PAGE. | |
| Amino acid sequence | |
| MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS. | |
| Biological Activity | |
| The activity as calculated by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 1ng/ml corresponding to a specific activity of 1,000,000 units/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Fibroblast Growth Factor b in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| 1. Title: Fibroblast Growth Factor-2 Autofeedback Regulation in Pituitary Folliculostellate TtT/GF Cells Publication: Endocrinology 150.7 (2009): 3252-3258. Link: FGF 2 Mouse prospec publication 2. Title: The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs Publication: Cold Spring Harbor Protocols 2010.4 (2010): pdb-prot5416. Link: FGF-2 Mouse prospec publication | |
| Protein content | |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of FGF2 as a Reference Standard. | |
| Precautions | |
| FGF 2 Human, Thermostable is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P09038 ) | |
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