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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0068-10 | 7 days | 10 µg | $120.00 |
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| GRF0068-50 | 7 days | 50 µg | $290.00 | |||
| GRF0068-1 | 7 days | 1 mg | $2,016.00 |
Product Overview | |
| Name | FGF 2 Mouse |
|---|---|
| Description | |
| Fibroblast Growth Factor-Basic Mouse Recombinant | |
| Accession (Primary) | P15655 |
| Synonyms | |
| HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b, Fibroblast Growth Factor-basic, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2. | |
| Introduction | |
| FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| FGF-b was lyophilized from 1mg/ml solution after extensive dialysis against 10mM Na 2 PO 4 , pH 7.5 and 50mM NaCl. | |
| Stability | |
| Lyophilized Rat bFGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat FGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 95.0% as determined by SDS-PAGE. | |
| Amino acid sequence | |
| MPALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS. | |
| Biological Activity | |
| The ED 50 range as determined by the dose-dependant proliferation of NR6R-3T3 cells was found to be 0.903ng/ml, corresponding to a specific activity of 1.1x10 7 units/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Rat bFGF in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Precautions | |
| FGF 2 Mouse is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P15655 ) | |
References |
1. Title: Fibroblast Growth Factor-2 Autofeedback Regulation in Pituitary Folliculostellate TtT/GF Cells Publication: Endocrinology 150.7 (2009): 3252-3258. Link: FGF 2 Mouse prospec publication 2. Title: The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs Publication: Cold Spring Harbor Protocols 2010.4 (2010): pdb-prot5416. Link: FGF-2 Mouse prospec publication |
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