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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0070-2 | 7 days | 2 µg | $120.00 |
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| GRF0070-10 | 7 days | 10 µg | $290.00 | |||
| GRF0070-1 | 7 days | 1 mg | $8,320.00 |
Product Overview | |
| Name | FGF 21 Bovine |
|---|---|
| Description | |
| Fibroblast Growth Factor-21 Bovine Recombinant | |
| Synonyms | |
| Fibroblast growth factor 21, FGF-21. | |
| Introduction | |
| The FGFs are a family of more than 20 small (~17–26 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors (FGFRs) 1, 2, or 3. A fourth closely related tyrosine kinase receptor (FGFR4) was able to bind the FGFs but did not lead to a mitogenic response. FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal cell function and glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis. FGF-19, has been shown to cause resistance to diet-induced obesity and desensitization and to improve glucose and lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure. FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered white lyophilized powder. | |
| Formulation | |
| Lyophilized from PBS, pH 7.4. | |
| Stability | |
| Lyophilized FGF-21 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| HPIPDS SPLLQFGGQV RQRYLYTDDA QQTEAHLEIR EDGTVGGAAD QSPESLLQLK ALKPGVIQIL GVKTSRFLCQ RPDGALYGSL HFDPEACSFR ELLLEDGYNV YQSEAHGLPL HLPGNKSPHR DPAPRGPARF LPLPGLPPAP PEPPGILAPQ PPDVGSSDPL SMVGPSQGRS PSYAS. | |
| Biological Activity | |
| The ED 50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 µg/ml, corresponding to a specific activity of > 2.0 × 10 3 IU/mg in the presence of 5 µg/ml of rMuKlotho-β and 10 µg/ml of heparin. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-21 Human Recombinant sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| References | |
| Title: Fibroblast Growth Factor 21 Action in the Brain Increases Energy Expenditure and Sensitivity in Obese Rats Publication: Diabetes 59.7 (2010): 1817-1824. Link: FGF 21 prospec publication | |
| Precautions | |
| FGF 21 Bovine is for research use only and not for use in diagnostic or therapeutic procedures. | |
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