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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0212-20 | 7 days | 20 µg | $200.00 |
Select product before adding to cart
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| GRF0212-100 | 7 days | 100 µg | $600.00 | |||
| GRF0212-1 | 7 days | 1 mg | $2,880.00 |
Product Overview | |
| Name | Leptin Pufferfish |
|---|---|
| Description | |
| Leptin Pufferfish Recombinant | |
| Accession (Primary) | Q588G0 |
| Source | |
| Escherichia coli. | |
| Physical Appearance | |
| White lyophilized (freeze-dried) powder. | |
| Formulation | |
| The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. | |
| Stability | |
| Lyophilized Leptin Antagonist Quadruple Mutant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln. | |
| Biological Activity | |
| Leptin Quadruple Antagonist Mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized Leptin Antagonist Quadruple Mutant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Protein content | |
| Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | |
| Precautions | |
| Leptin Pufferfish is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( Q588G0 ) | |
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ADMEbio Team
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ADMEbio Team
