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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0264-5 | 7 days | 5 µg | $120.00 |
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| GRF0264-25 | 7 days | 25 µg | $290.00 | |||
| GRF0264-1 | 7 days | 1 mg | $2,880.00 |
Product Overview | |
| Name | MIF Human His N |
|---|---|
| Description | |
| Macrophage Migration Inhibitory Factor Human, Recombinant His Tag N-Terminus | |
| Accession (Primary) | P14174 |
| Synonyms | |
| Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF. | |
| Introduction | |
| The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| MIF-Protein was lyophilized from 10mM sodium phosphate buffer pH-7.5. | |
| Stability | |
| Lyophilized MIF-protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIF-protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLM AFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVY INYYDMNAANVGWNNSTFA. | |
| Biological Activity | |
| Human PBMCs were cultured with 0 to 1000ng/ml Human MIF. Production of IL-8 was measured via ELISA after 24 hours. The ED50 which was found to be 88-132ng/ml. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized MIF-Protein in sterile 18MΩ-cm H2O at a concentration between 0.1mg-1mg per 1ml. | |
| Precautions | |
| MIF Human His N is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P14174 ) | |
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ADMEbio Team
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