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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| GRF0309-2 | 7 days | 2 µg | $120.00 |
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| GRF0309-10 | 7 days | 10 µg | $290.00 | |||
| GRF0309-1 | 7 days | 1 mg | $5,616.00 |
Product Overview | |
| Name | PDGF BB Mouse |
|---|---|
| Description | |
| Platelet-Derived Growth Factor BB Mouse Recombinant | |
| Accession (Primary) | P31240 |
| Synonyms | |
| Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Pdgfb, SIS, c-sis. | |
| Introduction | |
| PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. | |
| Source | |
| Escherichia Coli. | |
| Physical Appearance | |
| Sterile Filtered White lyophilized (freeze-dried) powder. | |
| Formulation | |
| PDGF-BB was lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS, pH 7.0. | |
| Stability | |
| Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
| Purity | |
| Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | |
| Amino acid sequence | |
| SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT. | |
| Biological Activity | |
| The ED 50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10 5 IU/mg. | |
| Solubility | |
| It is recommended to reconstitute the lyophilized PDGF-BB in sterile 18M-cm H 2 O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | |
| Precautions | |
| PDGF BB Mouse is for research use only and not for use in diagnostic or therapeutic procedures. | |
Target Information: ( P31240 ) | |
Background |
Platelet-Derived Growth Factor BB (PDGF-BB) is one of the isoforms of the PDGF family that plays a significant role in embryonic development, cell proliferation, cell migration, and angiogenesis. It's also relevant in the context of certain pathologies like cancer or healing processes. Here's what a research paper on Mouse Recombinant PDGF-BB might involve: Basic Biology: It might discuss the basic biology and function of PDGF-BB in the mouse model. This could include its role in various cellular processes like cell growth, division, and survival, as well as in the context of tissue repair or angiogenesis. Recombinant PDGF-BB Production: The paper might describe methods for producing recombinant PDGF-BB in laboratory conditions. It might go into detail on the expression system used (e.g., bacterial, yeast, or mammalian cells), the purification process, and how the functionality of the produced PDGF-BB was confirmed. Applications: It could also detail the experimental applications of recombinant PDGF-BB in the mouse model. For instance, it could be used to study wound healing processes, tissue regeneration, or the development and progression of certain diseases. Comparative Analysis: The research might include a comparison of the recombinant PDGF-BB with its natural counterpart in terms of functionality, structure, or other properties. Innovation: If the paper is presenting new research, it might discuss an innovative use of PDGF-BB, a novel production method, or new findings about its function. |
References |
1. Title: Analysis of fast protein phosphorylation kinetics in single cells on a microfluidic chip Publications: Lab on a Chip (2015) Link: PDGF BB Mouse prospec publication 2. Title: The Mouse Cornea as a Transplantation Site for Live Imaging of Engineered Tissue Constructs Publication: Cold Spring Harbor Protocols 2010.4 (2010): pdb-prot5416. Link: PDGF B-chain prospec publication |
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